We announce the establishment of a database for the ampc locus in a. Headtohead comparison of two multilocus sequence typing. The ability of this organism to gain multiple virulence factors. The name of the folder is the scheme name, say saureus for staphylococcus aureus. Each mlst scheme exists in a folder withing the mlstdbpubmlst folder. Acinetobacter baumannii is an important human pathogen usually associated with severe hospitalacquired infections. Characterization of carbapenemresistant acinetobacter. This server controls the allelic nomenclature of core genome mlst bacterial gene schemes. Comparative genomic analysis of acinetobacter baumannii. Investigation of a possible outbreak of carbapenem. Backgroundacinetobacter baumannii is a notorious opportunistic pathogen mainly associated with hospitalacquired infections. Multilocus sequence typing mlst databases and software. Acinetobacter outbreaks involving multidrugresistant strains have occurred worldwide 5, 6, 14, 28, 36, 39, 46, 47.
Carbapenem resistance in acinetobacter baumannii is most often encoded by oxacillinases oxas and less frequently by metallo. The population structure of acb has been studied using two 7gene mlst schemes, introduced by bartual and coworkers oxford scheme and by diancourt and coworkers pasteur scheme. The centers for disease control and prevention cdc recognizes multidrug resistant mdr a. Icaacicc 2015 september 17 21, 2015 san diego, ca san. This is commonly referred to as the oxford scheme because it was hosted on the pubmlst site at the university of oxford. Acinetobacter baumannii can cause serious infections in the lungs, blood, and brain. Acinetobacter baumannii becoming a superbug increasing carbapenem resistance among acinetobacter species is particularly troubling, because it is very often associated with multidrug resistance and because it is occurring in the context of increases in the incidence of acinetobacter infection 772017 dr. Multilocus sequence typing mlst analysis applied maths. Acinetobacter baumannii is a nosocomial pathogen increasingly affecting the critically ill patients and represents a major public health challenge. Acinetobacter baumannii update linkedin slideshare. This strain spread rapidly and caused a large outbreak in the hospital. The complete genome and phenome of a communityacquired acinetobacter baumannii. A new set of primers for rpob gene amplification is require to include a. A cgmlst scheme is a fixed and agreed upon number of genes for each species or group of closely related species that is ideally suited to.
To compare the two acinetobacter baumannii multilocus sequence typing mlst schemes and to assess their suitability to aid in outbreak analysis we investigated the molecular epidemiology of 99. Identification of acinetobacter species and genotyping of. Jul 07, 2017 acinetobacter baumannii becoming a superbug increasing carbapenem resistance among acinetobacter species is particularly troubling, because it is very often associated with multidrug resistance and because it is occurring in the context of increases in the incidence of acinetobacter infection 772017 dr. Development of a multilocus sequence typing scheme for characterization of clinical isolates of acinetobacter baumannii. Clonal diversity of acinetobacter baumannii clinical isolates.
Shortread wholegenome sequencing was conducted to determine the genetic basis of their antimicrobial resistance phenotype. Emergence of carbapenemresistant acinetobacter baumannii. Molecular epidemiology of carbapenemresistant acinetobacter. This tool will allow the molecular surveillance of nonclassic pathogens other than a. Dendrogram was generated by bionumerics software with the unweighted.
This site hosts publicly accessible multilocus sequence typing mlst databases and software. The aim of the present study was to characterise the acinetobacter isolates recovered from market meat samples in peru. Molecular epidemiology of clinical acinetobacter baumannii. It may also cause urinary tract and wound infections. An acinetobacter baumannii infection is caused by the acinetobacter baumannii bacteria germ. To better understand the genetic variation and antibiotic resistance mechanisms of.
Clinicians should be alert for this strain because its spread would have serious health consequences. Comprises fortyeight distinctive alleles of the ampc locus. This is commonly referred to as the oxford scheme because it was hosted on the pubmlst site at the. Due to its rapid ability to develop multidrug resistance mdr, a. Inaccurate multilocus sequence typing of acinetobacter. It is typically a short, almost round, rod shape coccobacillus. One clone was unique and the other was ampicillin susceptible. The platform of acinetobacter baumannii mlst databases provides an open access to all the data. A snapshot study was performed to investigate the clonal relatedness of a. Acinetobacter species assigned to the acinetobacter calcoaceticusbaumannii acb complex, are gramnegative bacteria responsible for a large number of human infections. Complete genome sequence of multidrugresistant acinetobacter baumannii strain 16562, which forms sturdy biofilm. In february 2006, a patient colonized with a multidrugresistant sequence type 56 acinetobacter baumannii strain was admitted to a hospital in madrid, spain.
In this study a multilocus sequence typing mlst scheme for acinetobacter baumannii was developed and evaluated by using 40 clinical a. These databases host mlst schemes and isolate data, increasingly including whole genome sequences. The database is openly accessible at the pubmlst platform. Acinetobacter baumannii is a notorious opportunistic pathogen mainly. To compare the two acinetobacter baumannii multilocus sequence typing mlst schemes and to assess their suitability to aid in outbreak analysis we investigated the molecular epidemiology of 99 acinetobacter baumannii isolates representing outbreakrelated and sporadic isolates from 24 hospitals in four different countries germany, poland, sweden, and turkey. Genomewide recombination drives diversification of. This site uses two linked databases powered by the bigsdb genomics platform. To further expand the limited multilocus sequence typing mlst database for acinetobacter baumannii, 53 clinical isolates from various outbreaks in europe and the usa, collected between 1991 and 2004, plus the a. Development of a multilocus sequence typing scheme for characterization of.
This study used a diverse collection of epidemiologically unrelated acinetobacter baumannii isolates to compare the robustness of a multilocus sequence typing mlst scheme, based on conserved regions of seven housekeeping genes, glta, gdhb, reca, cpn60, rpod, g yrb, and gpi, with that of sequencebased typing of bla oxa51like genes sbt bla oxa51like. The hosting of both schemes within a single bigsdb database, which can. It is urgently necessary to search for alternatives to conventional antibiotics for control of severe a. Acinetobacter baumannii is a globally distributed nosocomial pathogen that has gained interest due to its resistance to most currently used antimicrobials.
Acinetobacter baumannii is a typically short, almost round, rodshaped coccobacillus gramnegative bacterium. Multidrugresistant acinetobacter baumannii harboring oxa24 carbapenemase, spain joshi acosta, 1 maria merino, esther viedma, margarita poza, francisca sanz, joaquin r. Acinetobacter baumannii is a bacterial species that can survive on moist and dry surfaces. A total of 10 clinical samples were collected from 5 patients who were involved in the outbreak. Genome sequence of oxa23 producing acinetobacter baumannii. Acinetobacter is a genus of gramnegative bacteria belonging to the wider class of gammaproteobacteria. The isolates covered the time period of the study between 2006 and 2014 such that one isolate was from the year 2006, two were from the year 2007, one was from the. Complete genome analysis of three acinetobacter baumannii clinical isolates in china for insight into the diversification of drug resistance elements.
Aug 16, 2011 acinetobacter baumannii is an emerging human pathogen and a significant cause of nosocomial infections among hospital patients worldwide. The platform of acinetobacter baumannii mlst databases provides an open access to all the data and allows submissions of novel sequences. Acinetobacter baumannii is an emerging human pathogen and a significant cause of nosocomial infections among hospital patients worldwide. Development of a multilocus sequence typing scheme for. Acinetobacter baumannii efflux pumps and antibiotic resistance seyyed naser abdi,1,2 reza ghotaslou,3 khudaverdi ganbarov,4 ahmad mobed,1 asghar tanomand,5 mehdi yousefi,3 mohammad asgharzadeh,6 hossein samadi kafil1 1drug applied research center, faculty of medicine, tabriz university of medical sciences, tabriz, iran.
Identification and characterization of capsule depolymerase. It can be an opportunistic pathogen in humans, affecting people with compromised immune systems and is becoming increasingly important as a hospital derived infection nosocomial. Acinetobacter baumannii mlst pasteur database pubmlst. Acinetobacter baumannii is an important nosocomial pathogen that poses a serious health threat to immunecompromised patients. Acinetobacter baumannii has been a dreadful problem for icu physicians for a long time. Genomic analysis of antibiotic resistance for acinetobacter. We provide means of accessing these databases in common formats suitable for easy linking and importation to local resources. Species of the acinetobacter calcoaceticusacinetobacter baumannii complex are important human pathogens which can be recovered from animals and food, potential sources for their dissemination. Studies on the clonal relatedness of isolates could lay the foundation for effective infection control. Molecular epidemiology of clinical acinetobacter baumannii and. While we were isolating bartonella quintana from body lice, 40 acinetobacter baumannii strains were also isolated and genotyped.
Bacteremic pneumonia bp caused by this organism has a higher mortality compared to other organisms. This germ may be found on skin or in food, water, or soil. Interestingly, the sequence type of the first isolated 5. Characterization of epidemiologically unrelated acinetobacter. Acinetobacter species assigned to the acinetobacter calcoaceticus baumannii acb complex, are gramnegative bacteria responsible for a large number of human infections. Jul, 20 acinetobacter baumannii acinetobacter baumannii is a gram negative bacteria. Meningitis research foundation mrf has commissioned the creation of a comprehensive online library of the genomes of all meningococcal disease isolates for 201011 in england, wales and northern ireland.
Phylogenetic analysis revealed that ihit7853 belonged to the globally distributed international clone ic1 and mlst type st1st231 pasteuroxford mlst scheme. To compare the two acinetobacter baumannii multilocus sequence typing mlst schemes and to assess their suitability to aid in outbreak analysis we. This study aimed to analyze the clinical characteristics of patients and molecular mechanisms of the first outbreak mainly caused by sequence types sts 208 multidrug resistant mdr acinetobacter baumannii in china. Jan 30, 2020 each mlst scheme exists in a folder withing the mlst dbpubmlst folder.
Database for the ampc alleles in acinetobacter baumannii. In hospitalized patients, acinetobacter baumannii frequently colonizes. Relatedness determinations were carried out as previously described. Acinetobacter baumannii infection discharge care what. Multidrugresistant acinetobacter baumannii harboring oxa. Otero, fernando chaves, and german bou in february 2006, a patient colonized with a multidrugresistant sequence type 56 acinetobacter baumannii strain. The genus acinetobacter consists of 22 nomenspecies and at least 11 additional putative species. Full text acinetobacter baumannii efflux pumps and. Acinetobacter baumannii an update linkedin slideshare. The acinetobacter baumannii global clone 1 isolate ab3070294, recovered in the usa in 1994, and the global clone 2 gc2 isolate acicu, isolated in 2005 in italy, were among the first a.
Investigation of a possible outbreak of carbapenemresistant. The enormous increase in multidrug resistance among hospital isolates and the recent emergence of pandrugresistant strains underscores the urgency to understand how a. It can be an opportunistic pathogen in humans, affecting people with compromised immune systems, and is becoming increasingly important as a hospitalderived infection. Draft genome sequences of acinetobacter baumannii isolates. Bacterial identification and antibiotic sensitivity tests were performed.
In recent years, bacteriophages and their derivatives, such. This study used a diverse collection of epidemiologically unrelated acinetobacter baumannii isolates to compare the robustness of a multilocus sequence typing mlst scheme, based on conserved regions of seven housekeeping genes, glta, gdhb, reca, cpn60, rpod,gyrb, and gpi, with that of sequencebased typing of bla oxa51like genes sbtbla. Escherichia coli, salmonella enterica, klebsiella pneumoniae, campylobacter, acinetobacter baumannii, pseudomonas aeruginosa or any other organism available from an online repository, simply by. Acinetobacter baumannii acinetobacter baumannii is a gram negative bacteria. Mlst analysis in easy database setup an mlst database can be easily set up based on any publicly available mlst scheme definition for e. St208, st218, st231, st348, and two new sequence types suggesting that more than one clone is circulating palestinian hospitals. Shortread wholegenome sequencing was conducted to determine the genetic basis. Pdf genome sequence of acinetobacter baumannii tyth1. Phylogenetic and genomic diversity in isolates from the. Multidrugresistant acinetobacter baumannii harboring oxa24. Carbapenemnonsusceptible acinetobacter baumannii of.
Acinetobacter baumannii is a troublesome opportunistic pathogen with a high capacity for clonal dissemination. Multilocus sequence typing analysis of carbapenemresistant acinetobacter baumannii in a chinese burns institute acinetobacter baumannii is a leading pathogen responsible for nosocomial infections. Pcr and sequencing confirmed that bla oxa23 was the only acquired carbapenemase gene in all isolates. Apr, 2016 clonal dissemination is characteristic of the important nosocomial pathogen acinetobacter baumannii, as revealed by previous multilocus sequence typing mlst studies. To assess the possibility of using the mlst scheme for acinetobacter genomic species tu, separate analyses were performed for a. Here, we announce the draft genome sequences of two livestockassociated isolates recovered from sewage water from a poultry slaughterhouse in germany. Inaccurate multilocus sequence typing of acinetobacter baumannii. Pathogenic acinetobacter species including the novel. Comparative analysis of the two acinetobacter baumannii. Oct 14, 2015 acinetobacter baumannii is a globally distributed nosocomial pathogen that has gained interest due to its resistance to most currently used antimicrobials. Introduction acinetobacter baumannii is a gramnegative, nonfermentative, oxidasenegative coccobacillus. Background the emergence of multidrug or extensively drugresistant acinetobacter baumannii has made it difficult to treat and control infections caused by this bacterium.
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